Hi there
This Blog-Entry serves to answer several
questions regarding in-vitro culturing techniques based on the scientific
paper: Micropropagation of a difficult-to-root weeping mulberry (Morus alba var. Shidareguwa): A popular
variety for ornamental purposes (Aroonpong, 2015).
- Whenever plants are requested for agricultural
or pharmaceutical means, its cultivation is of economic importance. To improve
the belonging work steps and even the result, detailed knowledge is necessary. The
aim of the Mulberry-Study is to receive plants with higher rooting ability
after propagation.
- To carry out a successful shoot induction,
healthy, 3-year-old plants from the field are required. After shoot multiplication
on media, the root induction comes at a different media with 1-month-old
regenerated shoots (tips and nodes).
-
The results concerning the success of different
explant types are shown in Figure 2. It’s evident that shoot tips have the
highest survival rate and are much less affected of endo- and exophytic
contamination. In addition, there were no browning to proof after 5 days of
culture in this study.
-
The duration of the different acclimation stages
is of great importance. In vitro plants require more time generally than ex
vitro, to receive a survival rate from 90 – 100 %. Crucial factors are at one
hand the room air with its specific gas composition and at the other hand the
relentlessness of direct sun light.
Another interesting method to enhance the root
ability of plants or var. Shidareguwa in special is to breed instead of in
vitro cultivation. By taking a strong rooting variety as breeding partner the
new born shoots will show aspects of both parental plants (weeping character and
strong rooting).
Read more: Inheritance of a weeping character and the low
frequency of rooting from cuttings of the mulberry variety ‘Shidareguwa’ (H. Yamanouchi, 2009)
bye
